《植物生理学报》 2012, 48(1): 63-66

盐酸胍对毕氏海蓬子类囊体膜蛋白光谱特征的影响

周峰^1,*, 华春¹, 顾祝军¹, 秦晓春²

1南京晓庄学院生物化工与环境工程学院, 南京211171; 2中国科学院植物研究所光合作用与环境分子生理学重点实验室, 北

通信作者：周峰；E-mail: zfibcas@163.com；Tel: 025-86178262

摘　要：

研究了盐酸胍(GuHCl)处理对毕氏海蓬子类囊体膜蛋白亚基和光谱特征的影响。结果显示, 随着GuHCl处理浓度的增高, 类囊体膜蛋白的吸收光谱和荧光发射光谱明显下降, 峰位发生蓝移。这表明GuHCl处理下, 类囊体膜蛋白色素微环境发生明显变化, 色素蛋白结构遭到破坏; 较低浓度2 mmol·L-1 GuHCl处理下, 随着GuHCl处理时间的延长, 类囊体膜蛋白的吸收光谱和荧光发射光谱也呈下降趋势, 但与GuHCl浓度梯度处理比, 下降程度略缓, 峰位也基本没有变化。这说明类囊体膜在2 mmol·L-1 GuHCl不同处理时间下表现出的耐受性比在GuHCl浓度梯度处理条件强。

关键词：盐酸胍; 类囊体膜; 光谱

收稿：2011-11-12   修定：2011-12-10

资助：国家自然科学基金(41071281和30900095)、江苏省“青蓝工程”优秀青年骨干教师培养项目(2010)和南京市高等学校环境科学重点学科建设项目(2011)。

Effect of GuHCl on Spectra Characteristic of Thylakoid Membrane Protein of Salicornia bigelovii Torr.

ZHOU Feng^1,*, HUA Chun¹, GU Zhu-Jun¹, QIN Xiao-Chun²

¹School of Biochemical and Environmental Engineering, Nanjing Xiaozhuang University, Nanjing 211171, China; ²Key Laboratory of Photosynthesis and Environmental, Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China

Corresponding author: ZHOU Feng; E-mail: zfibcas@163.com; Tel: 025-86178262

Abstract:

The effects of GuHCl on protein subunit and spectra characteristic of thylakoid of Salicornia bigelovii were studied in this paper. The result showed that the absorption spectra and fluorescence spectra decreased significantly, and the peak position blue-shifted with increase concentration of GuHCl. It demonstrated that the microenvironment of thylakoid was changed and the structure of thylakoid was destroyed under GuHCl treatment. The absorption spectra and fluorescence spectra also decreased under prolongation treating time of 2 mmol·L-1 GuHCl. In contrast, the decrease extent of absorption spectra and fluorescence spectra was smaller than that of under different GuHCl concentration gradient treatment. Moreover, the peak position was almost not changed under 2 mmol·L-1 GuHCl treatment. It indicated that the tolerance ability of thylakoid under different time treatment of 2 mmol·L-1 GuHCl was higher than that of under different GuHCl concentration gradient treatment.

Key words: GuHCl; thylakoid; spectra